- Q What is the ratio of developers and testers?
- Q The ferric chloride test is used to determine the presence or absence of phenols in a given sample and it is not specific for hydrolysable tannins and will detect many nonpolymeric phenolics A specific test for condensed tannins is the production of anthocyanins following Butanol/HCl hydrolysis [Add 0.5 ml aq extract (preferably not containing any pigments) in a large screw cap tube, add 3.5 ml butan-1-ol/concHCl (v/v 95:5) and boil for 60 mins]. If solution turns red or purple then the extract contains condensed tannins. They also give pink colour with vanillin/1M HCl and a blue colour with DMACA (p-dimethylaminocinnamaldehyde). On the other hand, Bradford’s protein-binding dye, Coomassie brilliant blue G-250, forms an intensive blue-colored complex with hydrolysable tannins. If you can confirm condensed rather than hydrolysable tannins, I have a coulum purification method which has worked well with tannins from many different species. Can you say which species your extract is from?
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