Question
What information you get from the retardation factor value?
Answers
Retardation factor Rf is a measure of the separation of a particular component. It is expressed as
Rf = distance moved by the component spot/ distance moved by solvent front
Rf is a unit less quantity and lies between 0and 1.A value of 0 indicates no separation has taken place and 1 represents that the component has moved entire length alongwith the solvent front. In case two spots have same value of Rf it indicates that they are not resolved. At least a difference of 0.05 is necessary to discern the separation between two spots.   Your Comment




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